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Molecular Biology Of The Cell 6th Edition by Bruce Alberts -Test Bank

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  • ISBN-10 ‏ : ‎ 9780815344322
  • ISBN-13 ‏ : ‎ 978-0815344322

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Molecular Biology Of The Cell 6th Edition by Bruce Alberts -Test Bank

MOLECULAR BIOLOGY OF THE CELL, SIXTH EDITION
CHAPTER 9: VISUALIZING CELLS
© Garland Science 2015

1. If an average globular protein was of the size of a tennis ball, a typical animal cell would be as large as …
A. a cubicle.
B. a room.
C. a tennis court.
D. a stadium.
E. a city.

2. In the diagram below, a logarithmic scale of sizes is shown. Indicate which of the sizes indicated (A to H) better corresponds to the dimensions of each of the following. Your answer would be a three-letter string composed of letters A to H only, e.g. HCG.

 

( ) A bacterium
( ) An animal cell
( ) A globular protein

3. Indicate true (T) and false (F) statements below regarding light and light microscopy. Your answer would be a four-letter string composed of letters T and F only, e.g. FFFF.
( ) Two light waves of the same amplitude and frequency will completely cancel each other out if not perfectly in phase.
( ) If the refractive index of a medium is 1.1, light travels in a vacuum 1.1 times faster than it does in the medium.
( ) The limit of resolution for conventional light microscopy is approximately 0.4 µm, corresponding to the wavelength of violet light.
( ) A light-emitting particle can be detected with a light microscope even if it is several times smaller than the resolution limit of the microscope.

4. The following schematic diagram shows the path of light rays passing through in a light microscope. If the angular width of the cone of rays collected by the objective lens (2θ) is increased, would the resolution improve (I; i.e. the resolution limit decreases) or deteriorate (D)? Write down I or D as your answer.

 

5. In which of the following microscopy techniques are oblique rays of light focused on the specimen?
A. Bright-field microscopy
B. Dark-field microscopy
C. Phase-contrast microscopy
D. Differential-interference-contrast microscopy

 

6. The light used to excite a fluorescent molecule carries … energy and has a … wavelength compared to the light that is then emitted from the molecule.
A. greater; longer
B. greater; shorter
C. the same amount of; shorter
D. less; longer
E. less; shorter

7. Indicate whether you would use a fluorescent organic molecule (O), in situ hybridization (H), or a coupled fluorescent protein (P) to visualize the cells and their molecules in each of the following cases. Your answer would be a five-letter string composed of letters O, H, and P only, e.g. OHOOO.
( ) You would like to see where in the early Drosophila embryo the mRNA encoding a certain transcription regulator is located.
( ) You would like to see the nuclei and count them in an early mouse embryo.
( ) You would like to visualize chromosome 3 in a human cell culture derived from a patient’s tissue, based on specific sequences present on this chromosome.
( ) You would like to observe the oscillations in Ca2+ ions inside a fertilized frog egg.
( ) You would like to compare the localization of two transcription regulatory proteins in cultured human T cells.

8. What is the advantage of using quantum dots as an alternative to organic fluorochromes such as Cy3 and Alexa dyes?
A. They are brighter.
B. Their emission spectra can be precisely fine-tuned.
C. They have a longer lifetime and bleach more slowly.
D. All of the above.

9. Two approaches have been devised to deal with the problem of blurring in light microscopy with thicker samples. Indicate whether each of the following descriptions better applies to confocal design (C) or image deconvolution (D). Your answer would be a three-letter string composed of letters C and D only, e.g. CCD.
( ) It is normally faster.
( ) It requires a higher degree of sample illumination.
( ) It can be used to obtain images from relatively deeper parts of the specimen.

10. Which microscopy set-up uses a longer wavelength of light than usually excites a particular fluorophore? Which one allows researchers to peek deeper into biological samples?
A. Single-photon; single-photon
B. Single-photon; two-photon
C. Two photon; single photon
D. Two-photon; two-photon

 

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